[1]刘伯芹 王国峰 仲 玲 赵仁亮.bFGF和PDGF-B蛋白在脑缺血再灌注大鼠 模型中的表达和血管形成的研究[J].三才期刊采编系统演示平台,2015,1期(04):198-202.[doi:10.3969/j.issn.1007-0478.2015.04.002]
 Liu Boqin,Wang Guofeng,Zhong Ling,et al.The expression of basic fibroblast growth factor,platelet-derived growth factor B chain and its effects on angiogenesis in rats following cerebral ischemia/reperfusion[J].,2015,1期(04):198-202.[doi:10.3969/j.issn.1007-0478.2015.04.002]
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bFGF和PDGF-B蛋白在脑缺血再灌注大鼠 模型中的表达和血管形成的研究
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《三才期刊采编系统演示平台》[ISSN:1008-0341/CN:37-1280/R]

卷:
第1期
期数:
2015年04期
页码:
198-202
栏目:
论著
出版日期:
2015-12-31

文章信息/Info

Title:
The expression of basic fibroblast growth factor,platelet-derived growth factor B chain and its effects on angiogenesis in rats following cerebral ischemia/reperfusion
文章编号:
1007-0478(2015)04-0198-05
作者:
刘伯芹 王国峰 仲 玲 赵仁亮
266002 青岛市第九人民医院神经内科(刘伯芹 王国峰 仲 玲);
青岛大学附属医院神经内科[赵仁亮(通信作者)]
Author(s):
Liu BoqinWang Guofeng Zhong Ling et al.
Department of Neurology,Qingdao Ninth People's Hospital, Qingdao 266002
关键词:
碱性成纤维细胞生长因子 血小板源性生长因子-B 血管形成 脑缺血
Keywords:
Basic fibroblast growth factor Platelet-derived growth factor B chain Angiogenesis Cerebral ischemia
分类号:
R743
DOI:
10.3969/j.issn.1007-0478.2015.04.002
文献标志码:
A
摘要:
目的 探讨脑缺血再灌注大鼠不同时间碱性成纤维细胞生长因子(bFGF)和血小板源性生长因子-B(PDGF-B)的表达水平及其与血管形成的关系。方法 采用线栓法制备大鼠大脑中动脉局灶性脑缺血再灌注(MCAO/R)模型,分为假手术组和MCAO/R组,MCAO/R组缺血2 h后根据再灌注时间窗的不同分为0、6、24 h、3、7、14、21 d共7组,应用HE染色观察病理变化并测定脑梗死体积,用免疫组化法检测CD34蛋白的表达水平并计数微血管密度(MVD),用免疫组化法检测bFGF和PDGF-B蛋白的表达水平。结果 bFGF蛋白表达水平在MCAO/R 6 h后即开始升高,3 d到达高峰,7 d开始降低。PDGF-B蛋白表达水平在MCAO/R 24 h后明显升高,3 d到达高峰,7 d有所下降,持续到14 d左右。MVD表达在MCAO/R 3 d开始升高,7 d到达高峰。bFGF和PDGF-B蛋白表达水平与MVD变化呈正相关(P<0.01)。结论 局灶性脑缺血再灌注损伤可诱导bFGF、PDGF-B和新生血管表达增加,激活内源性脑保护机制。
Abstract:
ObjectiveTo investigate the effects of angiogenesis and the expression changes of basic fibroblast growth factor(bFGF)and platelet-derived growth factor B chain(PDGF-B )in rats following focal cerebral ischemia/reperfusion.Methods A model of the middle cerebral artery occlusion 2h / reperfusion(MCAO/R)in rats was performed with the intraluminal filament occlusion. Rats were divided into sham operation group and MCAO/R group. Two hours after ischemia the MCAO/R group was divided into 7 subgroups according to reperfusion time difference, including 0, 6, 24 h, 3, 7, 14, 21 d. Brain sections were stained with hematoxylin and eosin(HE)for surveying the volume of infraction. The expression of microvascular density(MVD)was determined by immunohistochemical staining. The expression of bFGF and PDGF-B protein were determined by mmunohistochemical staining.Results The expressions of bFGF began to increase at 6 h after ischemia/reperfusion. It reached climax at 3 d and reduced after 7 d. The expressions of PDGF-B were significantly increased at 24 h after ischemia/reperfusion, with the first peak at 3 d and the second peak was at 14 d. The expressions of MVD began to increase at 3 d after ischemia/reperfusion and continue to 14 d. There was a significant positive correlation between expression of bFGF, PDGF-B and change of MVD(P<0.05).Conclusions Ischemia and reperfusion injury can induce expression of bFGF, PDGF-B and angiogenesis, which might play an important role in the internal protection of neurons.

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备注/Memo

备注/Memo:
2014-12-24收稿
更新日期/Last Update: 2015-08-30