[1]任庆华 罗江兵 李长清.人参皂甙单体Rb1对大鼠脑缺血损伤 保护作用的实验研究[J].三才期刊采编系统演示平台,2015,1期(04):203-206.[doi:10.3969/j.issn.1007-0478.2015.04.003]
　Ren Qinghua,Luo Jiangbing,Li Changqing.Experimental study on the protection of ginseng Rb1 in rats with cerebral ischemic reperfusion injury[J].,2015,1期(04):203-206.[doi:10.3969/j.issn.1007-0478.2015.04.003]
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人参皂甙单体Rb1对大鼠脑缺血损伤 保护作用的实验研究
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《三才期刊采编系统演示平台》[ISSN:1008-0341/CN:37-1280/R]

卷:
第1期
期数:
2015年04期
页码:
203-206
栏目:
论著
出版日期:
2015-12-31

文章信息/Info

Title:
Experimental study on the protection of ginseng Rb1 in rats with cerebral ischemic reperfusion injury
文章编号:
1007-0478(2015)04-0203-04
作者:
任庆华 罗江兵 李长清
529000 广东省江门市人民医院神经内科(任庆华),神经外科(罗江兵);
重庆医科大学附属第二医院神经内科(李长清)
Author(s):
Ren Qinghua; Luo Jiangbing; Li Changqing
Department of Neurology, the People's Hospital of Jiangmen City,Guangdong Province, Jiangmen 529000
关键词:
脑缺血 人参皂甙 再灌注损伤 Rb1 NgR
Keywords:
Cerebral ischemia Ginseng Rb1 Reperfusion injury NgR
分类号:
R743
DOI:
10.3969/j.issn.1007-0478.2015.04.003
文献标志码:
A
摘要:
目的 观察人参皂甙单体Rb1 对大鼠实验性脑缺血的保护作用。方法 健康成年雄性清洁级SD大鼠随机分为假手术组(Sham)、缺血对照组(Con)、干预组(Tre),干预组又分为Rb130 mg/kg、60 mg/kg及90 mg/kg三个不同剂量组。缺血对照组采用线栓法建立大脑中动脉闭塞模型,缺血2 h后拨出线栓再灌注; 各干预组用相应剂量Rb1 ip qd×7 d,末次给药后3 h内用同样方法建立大脑中动脉闭塞模型及再灌注; 假手术组不插入线栓,余操作相同。术后48 h取标本TTC染色测梗死体积、干湿重法脑组织含水量测定、Tunnel法测定调亡细胞数及NgR表达的免疫组化测定。结果 各干预组的梗死体积分别为30 mg/kg组(27.629±1.401)%,60 mg/kg组(24.164±1.710)%,90mg/kg组(21.955±2.556)%,缺血对照组为(29.846±1.153)%; 脑组织含水量为30 mg/kg组(80.079±0.726)%,60 mg/kg组(78.984±0.902)%,90 mg/kg组(77.855±0.258)%,假手术组与缺血对照组分别为(76.517±0.37)%、(81.799±1.065)%; 调亡细胞数分别为30 mg/kg组(89.000±10.296),60 mg/kg组(59.000±12.522),90 mg/kg组(36.667±19.054),假手术组与缺血对照组分别为(1.600±1.517)、(132.667±22.223); NgR表达阳性面积分别为30 mg/kg组(84.827±3.870),90 mg/kg组(66.040±5.541),60 mg/kg组(75.577±7.150),假手术组与缺血对照组分别为(48.355±9.720)、(91.485±5.822)。结论 人参皂甙单体Rb1对大鼠实验性脑缺血有保护作用,能减轻缺血再灌注损伤所致的脑水肿及梗塞面积,减少细胞调亡,并且该保护作用呈剂量依赖性。对NgR表达的干预提示Rb1可能在脑缺血死后神经可塑性中起促进作用。
Abstract:
ObjectiveTo observe the protection of ginseng Rb1 in rats with local cerebral ischemic reperfusion injury.Methods Healthy Sprague-Dawley rats were divided into three groups: Sham, control and treat group. Treat group was divided into three subgroups: Rb130 mg/kg、60 mg/kg and 90 mg/kg group. MCAO models were created after seven days of Rb1 ip qd in treat group with respective dose and NS ip qd in control group.Tthe line extracted after occlusion 2 h. Sham group only receives sham operation. Rats were sacrificed at 48 h. The markers such as the expression intensity of NgR protein, brain water content, volume of infarction by TTC staining, and the count of apoptosis cell were estimated.Results Compared with control groups, the levels of expression of NgR protein, brain water content, volume of infarction and the count of apoptosis cell were lower in treat group and showed statistical difference(P<0.05). In treat group, there was also statistical difference between three subgroups.Conclusions The article show ginseng Rb1 has protective effect on rats with local cerebral ischemic reperfusion injury. Rb1 can decrease brain edema, infarction volume and apoptosis cell counting induced by local ischemic perfusion injury. It also indicates Rb1 may play a role in rehabilitation after CNS injury because Rb1 reduced the expression of NgR protein.

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备注/Memo

备注/Memo:
2014-09-18收稿
更新日期/Last Update: 2015-08-30